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TwistDx品牌/TwistAmp® nfo/用于末端检测,检测 DNA 复制反应
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发布时间:2025-07-09
TwistDx品牌/TwistAmp® nfo/用于末端检测,检测 DNA 复制反应
来源:默瑞(上海)生物科技有限公司2020-6-9 访问量:855评论(0)TWISTDX TwistAmp NFO kitsTwistDx品牌国内代理商--默瑞(上海)生物科技有限公司
TWISTDX TwistAmp NFO kits假阳性问题解答
Q:What will cause the false-positive results except DNA contamination?A:For the nfo kits another source of false positives is the dimerization of the two primers and in some cases the labeled primer, and the probe.When this happens you will have the biotin and FITC on an RPA product that will bind to the Lateral flow strip and bind to the ant-FITC gold particle that will result in a line in the test area on the Lateral Flow Strip.The primer sequences and probe sequence should be check for possible dimerization and they may need to be modified and screened.There are some basic rules for PCR primers that also hold true for RPA primers. Namely try to avoid:1) Long homo-polymer repeats2) Repetitive sequences3) Regions that could have lots of secondary structure4) High (>70%) GC or AT regions5)Sequences,likely,to,cause,primer-primer,interaction,(use http://www.thermoscientificbio.com/webtools/multipleprimer/to check this quickly)产品简介TwistAmp® 核酸扩增产品,能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低,特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp exo 用于real-time探针检测. 除基本组分外, 还含有内切酶3,减少扩增产物的末端拷贝量,所以不能用于电泳分析,但在RPA系统中产生更强的动态荧光信号,便于实时定量。产品详情介绍
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发布于 : 2025-07-09
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